ACE2 Protein, Human, Recombinant (His Tag)

重组人源ACE2蛋白(His标签)

 

●产品信息

种属:Human

来源:Eukaryotic expression

表达宿主:HEK293 Cells

纯度:> 95 % as determined by SDS-PAGE.

内毒素:<1.0 EU per μg protein as determined by the LAL method.

蛋白构建:A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with a polyhistidine tag at the C-terminus.

生物活性:Measured by its binding ability in a functional ELISA.

Immobilized 2019-nCoV Spike Protein (RBD, His Tag) at 2μg/mL (100μL/well) can bind human ACE2 protein (His tag),the EC50 of ACE2 is 20-90ng/mL

分子量:The recombinant human ACE2 consists of 734 amino acids and predicts a molecular mass of 85.1kDa.

预测N端:Gln 18

缓冲液:Sterile PBS, pH 7.4.(Please contact us for any concerns or special requirements.)

运输方式:Recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性&储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

 

●ACE2 Protein, Human, Recombinant (His Tag): SDS-PAGE图片

 

 

●ACE2 Protein, Human, Recombinant (His Tag):别称

ACEH Protein, Human

 

●ACE2背景信息

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.

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